Flowers and leaves extracts showed a powerful antimicrobial effect against all strains and the most. Pdf cytotoxic activity of a plant extract on cancer cells. Screening of invitro hypoglycemic activity of murraya koenigii and. The plant materials were air dried at room temperature for about 10 days and grounded into powder. The effect of plant extracts on the growth of wilt causing. Nov, 2014 the effect of plant leaf extract incorporated feed for the disease resistance survival percentage on fish n 20group were determined. Foundation and perspectives of the use of plant extracts as. A marked antimicrobial and antioxidant activity of leaf and flower extracts was observed. Phytochemical screening of some compounds from plant leaf. Potential genotoxic and cytotoxic effects of plant extracts.
After one hour at room temperature, the absorbance was measured at 420 nm. The antimicrobial activity of 12 plant extracts was tested against the phytopathogens alternaria alternata and fusarium solani. Evaluation of some plant extracts for their nematicidal properties against rootknot nematode, meloidogyne incognita m. Assessment of antioxidant activity of plant extracts by.
New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. Could it be inhibitory compounds in the plant extracts that inhibits cells growth. Syzygium cordatum mean 3 cfus had the lowest cfu reading at 40 minutes and hence was the. In order to extract different phenolic compounds from plants with a. Increasingly, natural products have potential for the treatment of cancer. Plant extracts generally contain flavonoids in glycosidic form. Phenolics are widespread constituents of plant foods fruits, vegetables. I have taken 100 seeds, the study were tested the antifungal activity of plant extracts and their effect of respective dilutions from plant extract with different plant extracts at 5.
The diluted working solutions of the test extracts were prepared in methanol. In this study we investigated of antibacterial and synergistic effect of ficus sycomorus. The extract from carrot was more heatstable than other extracts. In addition, the possible synergistic effects when associated with antibiotics were studied. Concentrationresponse curves for the cytotoxic effect of. Cytotoxic properties of some medicinal plant extracts from. Can a plant extract show activity at lower doseconcentration but not. The plants were inoculated with the dampingoff pathogen fusarium oxysporum and treatments were applied in a completely randomized design with a factorial arrangement of 4x2x3. Dry ethanolic extracts were obtained after removing the solvent by evaporation. Juss were evaluated for their growth, adult mortality and oviposition inhibition of c. This may be the reason why the plant extracts did not produce as marked inhibition as many of the pure compounds.
Plant phenolics are generally involved in defense against ultraviolet radiation or aggression by pathogens, parasites and predators, as well as contributing to plants colors. Evaluation of the cytotoxic activity of extracts from. Cytotoxic effects of bangladeshi medicinal plant extracts. Effect of humic acid and seaweed extracts on growth and yield of potato plant solanum tubersum l desiree cv. Dry powder was extracted with ethanol for about 7 days at room temperature. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. Phytochemical composition of ethanolic and aqueous extracts of the leaves, stembark, root, fruits and seeds i.
The study resulted in nonmutagenic and negligible cytotoxic plant extracts. The highest values of plant height, stem diameter, dry weight of leaves plant, leaf area, total carbohydrates and n contents were obtained with. Phytochemicals, antioxidant and antimicrobial potentials. To investigate the cytotoxic effect of some bangladeshi medicinal plant extracts, 16 bangladeshi medicinal plants were successively extracted with n hexane, dichloromethane, methanol and water. Cytotoxic and apoptotic effects of different extracts of. Biological screening of various medicinal plant extracts for. The results revealed that the extracts of the two plant species caused a considerable reduction in the number of weevils. Morphine was administered subcutaneously at a dose of 5mgkg body weight 10. Alcoholic or hydroalcoholic extracts from plant principles in which 1 ml of fluid extract is obtained from 1g of plant, e. They are ubiquitous in all plant organs and are therefore an integral part of the human diet. Pdf cytotoxic activities of certain medicinal plants on. In practice, plant extracts should be included in feeds at much higher doses to produce the same bactericidal or bacteriostatic effect observed in vitro. Antioxidant activity was determined by measuring the scavenging effect of plant extracts against 1, 1diphenyl2picryl hydrazyl dpph and using the ferric.
The growth inhibitory components were in the methylene chloride soluble fraction of the extract. Green plants extracts innovative botanical and herbal. Pdf potential genotoxic and cytotoxic effects of plant. Total phenolic content was found to be higher in leaf extracts except c. International journal of plant sciences, antifungal. Effect of some natural extracts on growth and chemical. The effect of various plant extracts was measured on several important oxidation indicators. The world health organization who estimates that up to. All plant extracts, except for tarragon, retarded peroxide formation at 80c the most effective being sage and sweet grass extracts fig. Antioxidant properties of the methanol extracts of the leaves. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. These activities have been corelated to the presence of certain phytochemical substances. Ten dif ferent plants were collected from in and around sivakasi area. Synergistic effect of ficus sycomorus moraceae leaf and.
Preliminary phytochemical screening, quantitative estimation. Therefore, in vivo, the mode and site of action of the active components of essential oils depend on their structure, metabolism, and inclusion level brugalli, 2003. Extraction, isolation and characterization of bioactive compounds. The present study deals with the analysis of phytochemical constituents by qualitative and quantitative analysis of moringa concanensis leaves, flowers and seeds were done using methanol extract. Dilution of stock solutions was made in culture medium yielding final extracts concentrations with a final dmso concentration of 0. This study evaluated the effect of growth substrates and plant extracts at different concentrations on germination and incidence of disease in pinus tecunumanii plants. A correlation has been observed between phenolic content of leaf and flower extracts and the antioxidant activity. The plant aqueous, ethanolic, acetone and methanolic extracts were screened for the presence of the phytochemical classes by using the standard following methods. In screening for genotoxic and cytotoxic effects, extracts of different plant parts have been used, ranging from leafy vegetables, fruits, and underground storage organs to whole plants. This concentration of dmso did not affect cell viability. Cytotoxic activity of extracts from plants of central. The current study included the preparation of the aqueous extract and alcohol leaves ziziphus ziziphus spina christi, eucalyptus plant. Nov 05, 20 the cells were treated with different concentrations of plants extracts including 0.
Antioxidant and in vitro cytotoxic activity of extracts of. Effect of aqueous and alcoholic plant extracts on inhibition of some. Antimicrobial and antioxidant activity of leaf and flower. The flower extracts of these two species were found to have higher cytotoxic effect on mcf7 cell with an ic50 value of 221. Pdf the anthelminthic efficacy of some differently obtained extracts of several plants was tested in vivo in laboratory animals and in vitro.
The antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method. Chemoprevention by natural products may be considered a promising approach to cancer control and management 1. Effect of crude plant extracts from some oaxacan flora on two. Evaluation of some plant extracts for their nematicidal. Herbs, spices, plant extracts and essential oils eo have been used for centuries to preserve food for humans and to make it more appetizing. The screening of plant extracts has been of great interest to scientists in the search for new everal diseases. The trees have some medicinal values as the sap extracted from the trunk. Pdf cytotoxic activities of extracts of medicinal plants. Effect of aqueous plant extracts and photosensitization on a. These data indicate that selected plant extracts are potential sources of dietary antioxidants. The cytotoxic effect of the mo extracts was dependent on the cell type. In vitro inhibitory activities of selected australian medicinal plant.
Saha 1department of agricultural chemistry, hajee mohammad danesh science and technology university, dinajpur, bangladesh. Now, the international to research plant active ingredients of cosmetics are in full swing, united states, japan, france, as well as some wellknown chinese cosmetic makers were add a variety of natural plant extracts in cosmetics, such as panax ginseng root extract, aloe vera extract, almond extract, polygonum multiflorum extract, ginkgo. Antiinflammatory and analgesic activity of methanolic. Herbs have a vital role in the prevention and treatment of cancer. In spite of this, the effectivity classification of the inhibition of phenolics and the extracts remained the same.
This value is the threshold established by the national cancer institute usa, nci plant screening program for an extract to be considered active following an incubation period of 48 and 72 h 52. The fish were artificially challenged with dose of 1. Alkaloids, flavonoids, terpenoids, carbohydrates, protein and amino acids were analysed. The aim of this study is to determine the antifungal activity and phytochemical composition of the plants extracts. The plant sources of india are likely to provide effective anticancer agents. The bioactive proteinpeptide extracts were found more free radical scavengers than the crude extracts of seeds and. Pdf the effects of different plant extracts on nematodes. Effects of medicinal plant extracts and photosensitization on. The present study aimed to assess the cytotoxic activity of extracts of selected plant species used for treatment of malaria in tanzania. Bello abstract phytochemicalsare active secondary plant metabolites responsible for most of the claimed medicinal activities of plants. A yellow color indicated the presence of flavonoids. The analysis of bioactive compounds present in the plant extracts involving. Pdf effects of herbal plant extracts on germination and. The effect was greater in the light than in the dark.
Baskaran abstract nematicidal activities of extracts from plants were assayed against meloidogyne incognita. Many studies have demonstrated antiproliferative, cytostatic and cytotoxic activities of phytochemicals against cancer cells 2. These products are well recognized for their health benefits in many societies around the world. Estimation of the total flavonoids in the plant extracts was carried out using the method of ordon ez et al. Cytotoxic studies of plant extracts on cancer cell lines zerumbone inhibited the proliferation of human colonic adenocarcinoma cell lines ls174t, ls 180, colo205 and colo320 dm in a dose dependent manner, while the growth of normal human dermal 2foc25 and colon ccd18 co fibroblasts was less affected. The leaf extracts of romatic plants, murraya koenigii and azadirachta a. Materials and methods the pure culture of the pathogen isolated from ripened. Antimicrobial effects of finnish plant extracts containing. Efficacy of plant extracts on the germination of wheat seeds.
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